Wararka

Dhuumaha waxaa caadi ahaan loo isticmaalaa shaybaarada si loogu gudbiyo qiyaasaha millilitir ee dareeraha ah, laga bilaabo ugu yaraan 1 ml ilaa ugu badnaan 50 ml. Cawska waxaa lagu tuuri karaa caag nadiif ah ama waxaa dib loogu isticmaali karaa muraayadda autoclavable. Labada pipette waxay isticmaalaan pipette si ay u damiyaan ugana saaraan dareeraha. Cabbirrada kala duwan ee pipettes ayaa loo isticmaalaa tijaabooyin kala duwan oo leh pipette isku mid ah. Tusaale ahaan, pipettes ayaa muhiim u ah isku dhafka xalalka kiimikaad ama ka joojinta unugga, ku wareejinta dareeraha weelal kala duwan, ama ku dhejinta reagents cufnaanta kala duwan. Ilaa iyo inta fiiro gaar ah loo yeesho mugga dareeraha la damiyay oo laga saaray, pipettes waxay noqon karaan qalab waxtar leh oo shaybaarka si sax ah loogu wareejiyo mugga millilitir ee dareeraha ah.

Noocyada pipettes iyo qaybaha aasaasiga ah ee pipettes

Dhuumaha inta badan waa tuubooyinka balaastikada ah ee aan nadiifka ahayn; Waxay sidoo kale noqon karaan autoclavable, tuubooyinka galaaska dib loo isticmaali karo.

Dhammaan pipette-yada waxay isticmaalaan pipette marka tuubooyinka.

pipette-ku wuxuu meesha ka saarayaa baahida loo qabo in cilmi-baarayaashu ay afka ka bixiyaan sidii hore. Habkaas dhuumaha qadiimiga ah laguma talinayo sababtoo ah waxay awood u leedahay inay keento cawaaqib xun oo dareere ah oo afka laga nuugo.

Kubbada pipette waa nooc ka mid ah pipette leh saxnaanta ugu xun. Badanaa waxaa lagu dhejiyaa pipette galaas ah si loogu wareejiyo qadarka dareeraha ah.

Bambooyinka pipette waxay sidoo kale ku habboon yihiin pipettes galaas ah, kuwaas oo wareejin kara qiyaaso dareere ah oo sax ah. Bambooyinka pipette guud ahaan waxay ku habboon yihiin in la bixiyo isla mugga dareeraha ah si isdaba joog ah.

pipettes-ka caawiyaha ayaa ah pipette-yada ugu caansan. Waxay ka kooban tahay dhowr qaybood oo waaweyn: af-mareenku waa meesha pipette la geliyo iyo meesha xuubka filterka la dhigo, kaas oo ka ilaaliya gudaha pipette kaaliyaha wasakhowga dareeraha.

Laba badhan ayaa lagu arki karaa gacanta tuubada caawiye. Marka badhanka sare la riixo, dareeraha waa la hamiyey, marka badhanka hoose la riixo, dareeraha ayaa soo baxaya.

Inta badan pipettes-ka caawiyaha waxa kale oo ay leeyihiin buro xakamaynta heerka dareeraha dareeraha ah. Tusaale ahaan, waxaa lagu dejin karaa in lagu sii daayo dareeraha cadaadiska, ama waxaa loo dejin karaa sii-deynta cuf-jiidka iyada oo aan lahayn xoog dibadda ah.

Halka qaar ka mid ah pipette-ka caawiyaha ay la yimaadaan fiilo koronto, inta badan waxay ku shaqeeyaan baytari.

Qaar ka mid ah pipettes kaaliyeyaasha waxay la yimaadaan istaajin ku habboon aagga gacanta, taas oo u oggolaanaysa pipette kaaliyaha in la dhigo dhinaceeda marka aan la isticmaalin iyada oo aan la saarin pipette.

Sida hore loo soo sheegay, pipette isku mid ah ayaa isticmaali kara cabbirro kala duwan oo ah pipettes iyadoo ku xiran mugga lagu shubayo, laga bilaabo 0.1 millilitir ilaa inta badan ilaa tobanaan millilitir.

Hawlgalka aasaasiga ah ee pipettes

Marka hore, dooro pipette cabbirka saxda ah iyadoo ku saleysan mugga dareeraha aad rabto inaad wareejiso. Ka dib xirmada ka fur xagga sare, taabo oo kaliya qaybta ka sarreysa calaamadda calaamadda, geli caarada pipette, oo ka saar baakidhka soo hadhay.

Marka xigta, ku qabso pipette hal gacan oo fur daboolka weelka uu ku jiro dareeraha aad rabto inaad damacdo. Adiga oo pipette-ku toosan, si tartiib ah u riix badhanka sare si aad si tartiib ah u hamiso shaybaarkaaga.

Isticmaal xariiqda qalin jabisay ee gidaarka pipette si aad u cabbirto mugga dareeraha aad rabto inaad wareejiso. Ogow in mugga waa in lagu akhriyo xagga hoose ee meniscus, ma aha in lagu akhriyo xagga sare.

Kadibna si taxadar leh ugu daa dareeraha weelka aad dooratay, adigoo ka taxadaraya in aanay caarada pipette taabto meel aan nadiif ahayn.

Si taxaddar leh u isticmaal si tartiib ah markaad saarayso dareeraha, gaar ahaan marka aad isticmaalayso tuubooyinka awoodda mugga yar, si aad uga fogaato wasakhaynta filtarka pipette kaaliyaha iyo muunada, ama waxyeello u geysato kaaliye pipette. Si xun u maamulida marka la isticmaalayo kaaliye pipette waxay ka cadhaysiin kartaa dadka kale ee khibrada u leh shaybaadhka, kuwaas oo laga yaabo inay kala soocaan pipette si ay u dayactiraan. Markaad bamgareyso xaddi badan oo dareere ah ama dareere soo daaya, xawaaraha wareejinta dareeraha waa la kordhin karaa adigoo si adag u riixaya badhanka.

Ugu dambeyntii, xasuuso inaad si habboon u tuurto cawska ka dib markaad wareejiso dareeraha.

Codsiga

Hadda oo aad ogtahay sida loo isticmaalo pipette, aynu si faahfaahsan u eegno qaar ka mid ah codsiyada shaybaarka caadiga ah.

Tallaabo muhiim ah marka la dhaqan gelinayo iyo dajinta unugyada waa qaybinta isku midka ah ee unugyada xalka ugu dambeeya. Ka joojinta unuggu si tartiib ah oo hufan ayaa loo qasi karaa iyadoo la isticmaalayo pipette, kaas oo isku mar isku daraya xalalka kiimikada iyo reagents.

Ka dib marka la go'doomiyo ama habaynta unugyada tijaabada ah, pipettes ayaa loo isticmaali karaa in lagu wareejiyo dhammaan clones-ka unugyada si loo ballaariyo ama falanqaynta tijaabada xigta.